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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial purpose in advertising and marketing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been claimed but specific nucleotide sequence details is not still readily available.
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Blocking peptides are peptides that bind precisely for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guidebook outlines further more possible optimization of mobile and immunoassay parameters to be sure the best click here possible results are...
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This manual outlines further more attainable optimization of mobile and immunoassay parameters to be certain the absolute best success are...
Antibodies certain to the blocking peptide now not bind towards the epitope over the target protein. By comparing the staining through the blocked antibody versus the antibody on your own, you can see which staining is unique.
Target Details Cyclin E belongs to your really conserved cyclin family members, whose associates are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which contribute towards the temporal coordination of each mitotic event. Cyclin E forms a posh with and features being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates on the G1-S section boundary and it is degraded as cells development by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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